This case study comes from a customer who volunteered to participate in our results program. In exchange for free testing, the customer agreed to use our five-day suppository regimen and then share their before-and-after lab results.
The customer’s main concern was a long-standing struggle with bacterial vaginosis (BV)—a recurring issue that had left her frustrated with conventional treatments. She was looking for a gentler, natural alternative that could still deliver measurable results.
The goal of the program was simple: to see whether a short, targeted course of probiotics, lactoferrin, and hyaluronic acid could measurably reshape the vaginal microbiome—and to confirm any changes with independent testing.
Two microbiome tests were performed through Juno Bio: one before starting the suppositories, and another seven days after completing the five-day protocol. This provided an unbiased look at how the microbiome shifted not just immediately, but after a stabilization period without ongoing supplementation.
In this case, a five-day course of probiotic and lactoferrin-based vaginal suppositories produced lab-confirmed changes. Even more impressive: results were verified after a seven-day waiting period with no supplementation, showing that the microbiome had stabilized naturally.
Starting Point: A Balanced but Watchful Microbiome
The first Juno Bio test, taken mid-July 2025, revealed a microbiome in relatively good shape. Protective bacteria were dominant, but the composition showed room for improvement:
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Lactobacillus bacteria: 70.75%
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Anaerobic bacteria (the main drivers of BV): 29.25%
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Aerobic bacteria: 0%
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Fungal load: None detected
This profile was considered Type 1, dominated by Lactobacillus crispatus—a community type associated with resilience against infection and inflammation. Still, the presence of nearly one-third anaerobic bacteria meant the environment wasn’t fully optimized. Anaerobes are known disruptors that can fuel bacterial vaginosis or recurrent irritation.
The Formula: A Multifunctional Approach
The suppositories were carefully designed, blending a natural cocoa butter base with highly targeted active ingredients. Each suppository contained:
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Probiotics (15 Billion CFU per dose):
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Lactobacillus crispatus
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Lactobacillus reuteri
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Lactobacillus acidophilus
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Lactobacillus rhamnosus
These strains are well-documented for their ability to acidify the vaginal environment, compete against pathogens, and restore balance.
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Lactoferrin: A natural milk protein with antimicrobial, antifungal, and antiviral properties. By binding iron, it limits nutrients available to harmful microbes, while also supporting local immune defenses.
Research shows lactoferrin can play a meaningful role in BV management: one clinical trial demonstrated that lactoferrin reduced recurrence and improved vaginal flora composition (Russo 2019), while another found that lactoferrin combined with probiotics restored healthy Lactobacillus levels more effectively than probiotics alone (Mastromarino 2014). Broader reviews also highlight lactoferrin as a key host defense molecule against microbial overgrowth (Valenti 2005).
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USP Hyaluronic Acid: Deeply hydrating, it soothes mucosal tissue, reduces irritation, and aids recovery of the epithelial lining.
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Organic Deodorized Cocoa Butter Base: Provides gentle delivery, moisturization, and gradual release throughout the night.
Together, these components addressed not just microbial imbalance but also hydration, comfort, and immune support.
The Five-Day Regimen
The protocol was simple yet structured:
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Duration: 5 nights in a row
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Timing: Before bed, for maximum overnight absorption
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Method: One suppository nightly, with a pantyliner to prevent leakage
By the end of the fifth night, the participant reported noticeable comfort improvements: less irritation, a more hydrated feeling, and no unusual discharge. Importantly, there was no rush to re-test right away. Instead, a seven-day consolidation period followed, giving the microbiome time to settle without ongoing supplementation.
The Results: Confirmed by Testing
The follow-up Juno Bio test, conducted on August 5, 2025, demonstrated striking changes:
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Lactobacillus bacteria increased from 70.75% → 81.17%
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Anaerobic bacteria (the main drivers of BV) decreased from 29.25% → 12.31%
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Aerobic bacteria appeared at 6.52%, specifically Enterobacteriaceae (including E. coli)
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Crispatus dominance strengthened from 41.49% → 56.97%
This shift is especially important because anaerobic bacteria are the primary culprits behind BV. They overgrow when Lactobacillus is low, leading to the recurring cycle of discomfort many women face. By cutting anaerobes by more than half while boosting Lactobacillus dominance, the five-day suppository regimen directly targeted the root cause of BV.
The one new finding—an uptick in aerobic Enterobacteriaceae—was flagged as worth monitoring, since these microbes are sometimes linked with urinary tract infections. However, the overwhelming Lactobacillus presence suggested a strong protective shield remained in place.
Why the Five-Day Reset Worked
1. Direct Delivery of Key Strains
Instead of relying on gut-to-vagina transfer (often inefficient with oral probiotics), intravaginal administration seeded protective strains exactly where they were needed.
2. Anaerobe Suppression Without Antibiotics
Antibiotics often wipe out good and bad bacteria alike. Here, anaerobes declined sharply while beneficial Lactobacillus flourished—a selective reshaping rather than a scorched-earth approach.
3. Immune & Tissue Support
Lactoferrin acted as a natural antimicrobial buffer, while hyaluronic acid supported hydration and tissue repair. This holistic support may have made the environment more hospitable for beneficial microbes.
4. Strategic Wait Period
By waiting seven days before re-testing, results reflected a truly stabilized microbiome—not an artificially boosted state from active supplementation.
The Enterobacteriaceae Question
The appearance of Enterobacteriaceae at 6.52% raised an eyebrow. Why would these bacteria surface after a positive intervention?
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Microbiome reshuffling: When anaerobes declined, ecological space opened that aerobes temporarily filled.
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Natural fluctuation: Vaginal microbiomes are dynamic; what shows up on one test may not persist.
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Host factors: Hormonal cycle, sexual activity, or hygiene habits may influence aerobic levels.
Given the sharp rise in Lactobacillus crispatus, the balance still tipped strongly toward protection. The likely outcome: with time and continued probiotic support, Enterobacteriaceae levels may naturally subside.
Key Takeaways
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Small, Targeted Interventions Can Have Big Results
Five days was enough to create measurable, positive change in the vaginal microbiome. -
Synergy of Ingredients Matters
The combination of probiotics, lactoferrin, and hydration support produced a more balanced, comfortable, and resilient ecosystem than probiotics alone could achieve. -
Testing Provides Validation
Subjective improvements were matched with objective lab-confirmed shifts, proving the intervention’s impact. -
Dynamic Systems Require Patience
The temporary rise in aerobes shows that the microbiome is a living system, adjusting and recalibrating over time.
A Blueprint for Women’s Health
This case highlights a new model of vaginal wellness:
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Short, manageable regimens that fit into everyday life
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Evidence-based formulations that combine microbiome science with supportive cofactors
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Testing before and after to validate results and guide ongoing care
For many women, this represents an empowering alternative to long-term antibiotics or guesswork-driven home remedies.
Conclusion
In just five days, with a simple nightly routine, the participant achieved meaningful microbiome improvements. By the time of the second test, Lactobacillus crispatus dominance had deepened, anaerobes had dropped significantly, and overall balance looked stronger than before.
While the appearance of Enterobacteriaceae underscored that microbiomes are never static, the results prove that fast, natural, and verifiable progress is possible.
For anyone seeking to restore balance and resilience, this case shows that sometimes less is more—five days of precise intervention can reshape the vaginal microbiome in ways that truly matter.
📚 References
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Russo R, Karadja E, De Seta F. Evidence of Lactoferrin Efficacy Against Bacterial Vaginosis. PubMed
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Mastromarino P, et al. Effects of Lactoferrin in Combination with Lactobacilli on Vaginal Flora in Women with Bacterial Vaginosis. PubMed
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Valenti P, Antonini G. Lactoferrin: An Important Host Defence Against Microbial and Viral Attack. PubMed