A Customer Exploration of Vaginal Microbiome Balance
This case study comes from a customer who volunteered to participate in our microbiome results program. In exchange for free microbiome testing, the participant agreed to use our five-day suppository routine and share her before-and-after lab results.
Many women are curious about how their vaginal microbiome responds to different products and routines. Our program was created to help customers explore these changes using independent microbiome testing.
The participant who shared this experience had spent years experimenting with different approaches to vaginal care and was interested in understanding how a short routine focused on microbiome support might influence her personal microbiome profile.
The goal of the program was simple: to observe whether a short, targeted course of probiotics, lactoferrin, and hyaluronic acid could shift the composition of the vaginal microbiome — and to document any changes through independent laboratory testing.
Two microbiome tests were performed through Juno Bio: one before starting the suppositories, and another seven days after completing the five-day routine. This timing allowed us to observe not only the immediate effects, but also how the microbiome looked after a short stabilization period without continued supplementation.
Starting Point: Understanding the Baseline
The first Juno Bio test, taken in mid-July 2025, showed a microbiome that was generally balanced but still evolving — something that is very common.
The results looked like this:
Lactobacillus bacteria: 70.75%
Anaerobic bacteria: 29.25%
Aerobic bacteria: 0%
Fungal load: None detected
This profile was categorized as Type 1, meaning it was largely dominated by Lactobacillus crispatus, a species commonly associated with a stable and resilient vaginal environment.
At the same time, the presence of a notable portion of anaerobic bacteria suggested there was still room for the ecosystem to shift further toward Lactobacillus dominance — something many women aim for when focusing on microbiome-friendly routines.
The Formula: Designed to Support the Microbiome Environment
The suppositories were designed to support multiple aspects of vaginal wellness at once, combining microbiome-focused ingredients with hydration and comfort support.
Each suppository contained:
Probiotics (15 Billion CFU per dose)
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Lactobacillus crispatus
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Lactobacillus reuteri
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Lactobacillus acidophilus
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Lactobacillus rhamnosus
These strains are commonly studied for their role in maintaining an acidic environment and supporting a Lactobacillus-dominant microbiome.
Lactoferrin
Lactoferrin is a naturally occurring protein found in milk and other body fluids. It has been widely studied for its role in supporting the body’s natural microbial balance and helping maintain a healthy microbial environment.
USP Hyaluronic Acid
Hyaluronic acid provides hydration and helps support mucosal comfort, which can be beneficial for maintaining a healthy tissue environment.
Organic Deodorized Cocoa Butter Base
The cocoa butter base allows the suppository to melt gradually overnight, helping distribute the ingredients gently while also providing moisturization.
Together, these components were designed to support hydration, microbial balance, and overall comfort.
The Five-Day Routine
The routine followed by the participant was intentionally simple.
Duration: 5 consecutive nights
Timing: before bedtime
Method: one suppository nightly
Using the product at night allows the ingredients to remain in place longer while the body is at rest.
After the five-day routine was completed, the participant waited seven additional days before retesting. This pause allowed the microbiome to stabilize naturally before the second sample was taken.
During this period, the participant reported subjective improvements in comfort and hydration.
What the Follow-Up Test Showed
The second Juno Bio test, performed on August 5, 2025, revealed noticeable shifts in the microbiome composition.
Lactobacillus bacteria increased from 70.75% → 81.17%
Anaerobic bacteria decreased from 29.25% → 12.31%
Aerobic bacteria appeared at 6.52%, specifically Enterobacteriaceae
Crispatus dominance increased from 41.49% → 56.97%
Overall, the microbiome moved toward a stronger Lactobacillus-dominant profile, which many researchers consider a hallmark of vaginal microbiome balance.
Interpreting the Microbiome Shift
One interesting observation was the temporary appearance of Enterobacteriaceae, a family of aerobic bacteria that sometimes appears during microbiome transitions.
Several factors may explain this:
Microbiome reshuffling
When certain microbial groups decrease, ecological space can temporarily be filled by others.
Natural variation
The vaginal microbiome naturally fluctuates over time.
Lifestyle and biological factors
Hormonal cycle timing, sexual activity, and daily habits can all influence microbiome composition.
Despite this temporary shift, the overall trend toward stronger Lactobacillus presence suggested a microbiome that had moved toward greater balance.
What This Customer Experience Suggests
While this is just one individual case, it offers a useful glimpse into how short, targeted routines may interact with the vaginal microbiome.
A few observations stood out:
Short routines can still produce measurable changes
The microbiome shifted noticeably after only five days.
Combination formulas may support multiple aspects of the environment
Probiotics, lactoferrin, and hydration support addressed different elements of vaginal wellness simultaneously.
Testing adds valuable insight
Independent microbiome testing allowed the participant to see objective changes rather than relying only on subjective impressions.
Microbiomes are dynamic systems
Even positive shifts may include temporary fluctuations as the ecosystem adjusts.
A New Way to Think About Vaginal Wellness
For many women, microbiome testing offers a way to better understand their bodies and how different products interact with their personal microbiome.
This case highlights a growing approach to vaginal wellness:
• short routines that are easy to incorporate into daily life
• microbiome-aware formulations
• optional testing to track changes over time
Rather than guessing which products may work best, microbiome insights allow women to observe how their own microbial ecosystem responds.
Final Thoughts
In this case, a simple five-day routine was followed by a measurable shift toward greater Lactobacillus dominance and overall microbiome balance.
While every microbiome is unique, this experience demonstrates how microbiome-focused routines and testing can provide valuable insight into vaginal wellness.
For many women, understanding their microbiome may become an important part of maintaining comfort, balance, and confidence in their daily lives.
📚 References
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Russo R, Karadja E, De Seta F. Evidence of Lactoferrin Efficacy Against Bacterial Vaginosis. PubMed
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Mastromarino P, et al. Effects of Lactoferrin in Combination with Lactobacilli on Vaginal Flora in Women with Bacterial Vaginosis. PubMed
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Valenti P, Antonini G. Lactoferrin: An Important Host Defence Against Microbial and Viral Attack. PubMed